Skip to main content
. Author manuscript; available in PMC: 2017 Jan 10.
Published in final edited form as: Dev Biol. 2016 Oct 4;420(1):136–147. doi: 10.1016/j.ydbio.2016.09.020

Fig. 8.

Fig. 8

Pharynx differentiation is required for transdifferentiation into intestine. (A) The pharynx (outlined in white) in a hatched worm from control (empty vector, L4440) RNAi conditions. (B) Pharynx region after pha-4-targeted-embryonic RNAi; all worms completely lack a pharynx structure (n=47). (C) Percentage of worms from control or pha-4 RNAi conditions with intestine-specific “gut granules” anterior to the normal intestine several days after ectopic ELT-7 expression. HS, heat shock. n, number of worms. (D–F) ELT-2 and IFB-2 expression in hatched worms 24 h after ectopic ELT-7 expression. Anterior is to the left and the white line marks the beginning of the intestine. Most worms lack ELT-2 or IFB-2 in the pharynx region after pha-4 RNAi (E–E’); however, a fraction of worms contain a small number of ELT-2- and IFB-2-positive cells in the pharynx region (F–F’). (G) Percentage of worms with ELT-2-positive nuclei in the region anterior to the normal intestine after control and pha-4 RNAi and ectopic ELT-7 expression. n, number of worms with immunoreactive nuclei.