Figure 5. Rsp5 is required for the degradation of cytosolic misfolded proteins.

a–d. Degradation of ³⁵S labeled proteins in WT (grey) and rsp5-1 (green) cells at 25°C (dotted lines) or upon HS (solid lines; 38°C in a, c and d and 45°C in b). The portion of proteins degraded after the chase at the indicated times was measured for short-lived (a, b) and long-lived (c) proteins. The portion of proteins that mainly correspond to cytosolic proteins degraded after the chase was measured at the indicated times in (d). All experiments were done in three independent experiments (average±SD; source values are listed in Supplementary Table 5).