Table 1.

Cell cycle related gene expression after FGF-2 and/or FGF-16 treatment in neonatal cardiac myocytes

Genes	Category	FGF-2	FGF-16	FGF-2 + FGF-16
Ccnf (cyclin F)	6	2.80*	1.26	1.83
Mki67 (Ki67)	2	2.22*	1.28	1.49
Arf/INK4A	5/7	1.25	1.20	2.38*

Categories of functional gene grouping: 1, G₁ phase and G₁/S transition; 2, S phase and DNA replication; 3, G₂ phase and G₂/M transition; 4, M phase; 5, cell cycle checkpoint and cell cycle arrest; 6, regulation of the cell cycle; 7, negative regulation of cell cycle (www.superarray.com). Cultures of neonatal cardiomyocytes were treated with vehicle, FGF-2 (1 ng/ml), FGF-16 (100 ng/ml), or FGF-2 combined with FGF-16 for 24 h, followed by extraction of total RNA. Real-time RT-PCR was carried out on a 96-gene mini array. Data are mean fold change of mRNA expression in treatment group vs. control from 3 separate experiments. Gene expression level, expressed as inverse relationship to threshold cycle (C_t) and doubling of amount of product with every cycle, is calculated using the equation L = 2^−Ct. After correction using housekeeping gene expression, relative expression level for each treatment group is given by [L(GOI)^experiment/L(HKG)^experiment]/[L(GOI)^control/L(HKG)^control], where L is gene expression level, GOI is gene of interest, and HKG is housekeeping gene average value. When calculated using L = 2^−Ct, relative expression is equivalent to 2^−ΔΔCt.

^*Numbers pass dual criteria where 1) gene expression level is >200% of control and 2) P < 0.01, when treatment is compared with control by repeated measures ANOVA and the Dunnett posttest for comparisons of multiple treatments to a common control.