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. Author manuscript; available in PMC: 2017 Jan 10.
Published in final edited form as: Cardiovasc Res. 2010 Jan 22;87(1):102–110. doi: 10.1093/cvr/cvq025

Figure 3.

Figure 3

(A) Hybrid mFGF-16p.luc genes with truncated regions of the upstream DNA flanking the ATG start codon containing either TATA1 or TATA2 alone, or TATA1 and TATA2 were used to transfect cardiomyocytes. Cultures were treated with or without IsP and luciferase activity measured at 24 h. (B) The −747/−12 mFGF-16p.luc gene containing TATA1 and putative NF-κB DNA elements (Figure 1) was tested for the effect of NF-κB inhibitors (PDTC and PS-1145) pretreatment on IsP responsiveness (6 h). Results for each hybrid gene in (A) and (B) are expressed relative to luciferase activity in untreated (Control) cultures, which has been arbitrarily set to 1.0. Control activity levels for −5771/−1039, −5771/−12, and −747/−12 mFGF-16p.luc are 16.20 ±0.03, 23.84 ±0.04, and 21.69 ±0.07, respectively, and are means ±SEM (n = 3–6).