Skip to main content
. Author manuscript; available in PMC: 2018 Jan 9.
Published in final edited form as: Curr Biol. 2016 Dec 1;27(1):1–15. doi: 10.1016/j.cub.2016.10.044

Figure 4. PQ Pre-treatment Induces Lon in a Male-specific Manner that Diminishes with Age.

Figure 4

(A,B) Control flies assayed for lon mRNA expression following PQ pre-treatment. lon mRNA in (A) 3 day and 60 day females. (B) 3 day and 60 day males. (C–F) Lon protein following PQ pretreatment. (C) 3 day females. (D) 3 day males. (E) 60 day females. (F) 60 day males. Western blots performed in triplicate, quantified using Image J. (G,H) Proteolytic capacity to degrade oxidized [3H] aconitase in presence of 5mM ATP measured in mitochondria from 3 day and 60 day control flies following PQ pre-treatment. (G) Females. (H) Males.