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. 2017 Jan 10;12(1):e0169544. doi: 10.1371/journal.pone.0169544

Fig 4. Expression of the cymAb and cmtAb genes during LB400 growth on p-cymene and p-cumate, and activity of p-cumate dioxygenase.

Fig 4

(A) Expression of cymAb and cmtAb genes during LB400 growth on p-cymene and p-cumate. Expression of cymAb (BxeA3559) and cmtAb (BxeA3556) genes in LB400 cells grown in glucose (lane 1), succinate (lane 2), p-cymene (lane 3), p-cumate (lane 4) and p-cymene plus succinate (lane 5). RT-PCR assays were performed using RNA from LB400 cells collected at exponential growth phase. The expression of 16S rRNA was used as a control to normalize across samples. At least three independent RNA samples were collected at each condition and two independent RT-PCR reactions for each sample were done to assess reproducibility. (B) Activity of the p-cumate dioxygenase CmtA in LB400 cells. LB400 cells grown overnight in LB medium and washed with M9 medium were incubated with 2.5 mM p-cumate and 0.3% lactate. Absorbance values are the mean ± SD of at least three independent experiments.