Table 3. Identification of LB400 proteins repressed during growth on p-cymene.
| Spot No.a | Gene | pI/Mr (kDa)b | Similarity (Organism) | Id (%) | Fold Change c |
|---|---|---|---|---|---|
| Stress response proteins | |||||
| 124 | BxeA3816 | 5.80 / 10.46 | Co-chaperonine GroES (Ralstonia pickettii 12D) | 91 | Glu |
| Transport and membrane proteins | |||||
| 10 | BxeA3152 | 6.29 / 19.70 | Fe2+ transport protein (Achromobacter xylosoxidans AXX-A) | 67 | -10.6354 |
| 17 | BxeA0391 | 9.48 / 24.14 | ABC transporter, periplasmic ligand binding protein, toluene tolerance (Cupriavidus metallidurans CH34) | 55 | -3.5158 |
| 27 | BxeB2189 | 8.61 / 28.23 | Lysine/arginine/ornithine ABC transporter (Chromobacterium violaceum ATCC 12472) | 75 | -2.1912 |
| 37 | BxeA0546 | 8.73 / 35.06 | Monosaccharide-transporting ATPase (Ralstonia pickettii 12J) | 69 | -2.3706 |
| 49 | BxeA0475 | 8.83 / 40.22 | Porin (Ralstonia eutropha H16) | 58 | -3.3821 |
| 47 | BxeA0050 | 8.82 / 40.39 | ABC transporter periplasmic protein (Ralstonia eutropha H16) | 71 | -2.0697 |
| 129 | BxeA4348 | 9.34 / 48.81 | Amino acid permease-associated protein (Ralstonia pickettii 12D) | 77 | Glu |
| 136 | BxeA0597 | 8.95 / 32.51 | ABC transporter periplasmic protein (Ralstonia eutropha H16) | 64 | Glu |
| 140 | BxeA3451 | 8.77 / 44.13 | Sugar ABC transporter substrate-binding protein (Burkholderia phytofirmans PsJN) | 72 | Glu |
| Aromatic metabolism | |||||
| 23 | BxeB2882 | 5.85 / 24.78 | N-(5'-phosphoribosyl)anthranilate isomerase (Ralstonia solanacearum UW551), biosynthesis tryptophan | 65 | -3.5449 |
| 34 | BxeC1192 | 5.28 / 28.90 | cis-2,3-dihydroxy-2,3-dihydrobiphenyl dehydrogenase BphB (Pseudomonas sp. Cam-1) | 99 | -3.3409 |
| Synthesis of proteins | |||||
| 127 | BxeA1009 | 10.52 / 14.20 | 50S ribosomal protein L19 (Ralstonia pickettii 12D) | 85 | Glu |
| Energy metabolism | |||||
| 19 | BxeA3400 | 5.30 / 19.24 | Inorganic pyrophosphatase (Cupriavidus metallidurans CH34) | 82 | -2.6932 |
| 132 | BxeA0590 | 6.20 / 22.18 | Bifunctional 4-hydroxy-2-oxoglutarate aldolase/2-dehydro-3-deoxyphosphogluconate aldolase (Acinetobacter sp. ADP1) | 84 | Glu |
| 138 | BxeA0566 | 6.60 / 36.20 | Glyceraldehyde 3-phosphate dehydrogenase (Ralstonia eutropha H16) | 85 | Glu |
| Second messenger synthesis | |||||
| 29 | BxeB2035 | 5.71 / 85.67 | Diguanylate cyclase/phosphodiesterase (Allochromatium vinosum DSM 180) | 37 | -1.7790 |
| PHA metabolism and fatty acid β-oxidation | |||||
| 12 | BxeA1544 | 6.18 / 20.09 | Phasin (PHA-granule associated protein) (Cupriavidus metallidurans CH34), PhaP1 | 74 | -4.9593 |
| 28 | BxeA0762 | 5.93 / 26.27 | 3-hydroxyacyl-CoA dehydrogenase (Ralstonia solanacearum GMI1000) | 79 | -3.8809 |
| Other functions | |||||
| 11 | BxeA4352 | 5.36 / 84.88 | Xanthine dehydrogenase, molybdenum binding subunit apoprotein (Ralstonia eutropha JMP134), purine metabolism | 83 | -4.2960 |
| 14 | BxeB1750 | 9.19 / 21.61 | Hypothetical protein [Pseudomonas protegens Pf-5] | 58 | -4.7280 |
| 31 | BxeA3631 | 8.76 / 25.34 | Periplasmic or secreted protein (Cupriavidus metallidurans CH34) | 56 | -1.7848 |
| 135 | BxeA4083 | 6.33 / 28.38 | GntR family transcriptional regulator (Bordetella petrii DSM 12804) | 53 | Glu |
a Spot number shown in Fig 5.
b Theoretical isoelectric point and molecular mass.
c Change in expression of the protein. Negative values refer to proteins repressed during growth on p-cymene as the sole carbon source, compared to glucose-grown cells Glu, only detected in glucose-grown cells.