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. Author manuscript; available in PMC: 2018 Jan 15.
Published in final edited form as: J Immunol. 2016 Nov 30;198(2):895–907. doi: 10.4049/jimmunol.1601448

Figure 2.

Figure 2

Induction of a tracheal mucosal inflammatory response to HDM allergen. (A) Percentages of T cells (blue) and eosinophils (red) from tracheal and lung tissue digests on days 2-12 following a single o.ph. treatment of C57Bl/6 mice with HDM/CT. Gated on live extravascular cells. Lung eosinophils were distinguished from alveolar macrophages based on their low autofluorescence in the green (AF 488) channel. (B) Confocal images showing T-cell clusters and associated eosinophils in a tracheal whole-mount at d9 after HDM/CT. Boxed region in left panel (bar=200μm) was enlarged (right panel, bar=20μm). (C, E, G) Comparison of lung and tracheal tissue digests from HDM/CT and PBS-treated mice. Gates indicate the CD4+ T-cell response at d8 (C), eosinophil and DC response at d10 (E) and neutrophil response at d8 (G). Numbers show percentages from the total live extravascular population. (D, F, H) Analysis of the tracheal inflammatory response developing between d6-d10. Total numbers of CD4+ T cells (D), eosinophils (F) and neutrophils (H) per trachea were determined using the gates shown in C, E and G. Graphs indicate mean ± SEM. n=2-3 mice per time point (in a single experiment) were analyzed.