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. Author manuscript; available in PMC: 2018 Jan 15.
Published in final edited form as: J Immunol. 2016 Nov 30;198(2):895–907. doi: 10.4049/jimmunol.1601448

Figure 5.

Figure 5

Both CD11b+ and CD11b− DCs form contact with cytokine-producing effector T cells in the tracheal mucosa. CD11c-EYFP mice were treated with HDM/CT on d0 and re-challenged with HDM on d7. On d9, perfusion-fixed tracheas were collected for whole-mount immunostaining. (A-D) Confocal microscopic analysis of interactions between IL-17+ T cells and CD11b+ (A, B) or CD11b− (C, D) DCs. Bars=20μm, arrows indicate IL-17+ T cells in contact with DCs. In C, double arrow shows a CD11b− DC, block arrow shows a CD11b+ DC. A and C show separate channels, B and D show the respective merged images as XY, XZ and YZ representations. In B and D, a range of slices is shown, as indicated by tick marks. (E) Quantitative analysis showing the contribution of CD11b+ vs. CD11b− DCs to forming conjugates with cytokine-positive T cells in the tracheal mucosa. A total of 68 IL-17+ T cells in contact with a CD11c-EYFP+ DC in tracheal whole mounts of 5 mice were analyzed. A total of 49 IL-13+ T cells in DC-contact in tracheal frozen sections of 5 mice were analyzed. (F) Relative frequency of CD11b− vs. CD11b+ DCs in the tracheal mucosa. Tracheal whole mounts of 5 mice were analyzed. Results of a single experiment are shown.