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. Author manuscript; available in PMC: 2018 Jan 15.
Published in final edited form as: J Immunol. 2016 Nov 30;198(2):895–907. doi: 10.4049/jimmunol.1601448

Figure 6.

Figure 6

Polyclonal “HDM-primed” T cells, but not OT-II cells arrest on DCs and produce effector cytokine. (A) Adoptive transfer and sensitization protocol. (B) Flow-cytometric analysis of tracheal tissue digests to compare the tracheal polyclonal CD4+ response in HDM/CT sensitized vs. control (PBS-treated) mice. Analysis of recipient mice was performed at d8 post-sensitization. Numbers indicate percentages. (C) Snapshot from Supplemental Video 6 (left panel, bar=100 μm) and representation of corresponding cell motility tracks (right panel). (D) Quantitative comparison of mean velocity and arrest coefficient of “HDM-primed” polyclonal vs. OT-II effector cells. Circles indicate individual cell tracks; horizontal white bars show mean values. Data were pooled from the analysis of three mice, which were performed as three independent experiments. (***:p < 0.001 and ****:p < 0.0001 as determined using Mann-Whitney test). (E) Whole-mount IL-17 staining of tracheas collected after MP-IVM analysis. Boxed region in left panel (bar=50μm) was enlarged (right panel, bar=10μm) to indicate IL-17 production by polyclonal T cells (arrow) but not by OT-II cells (double arrow). 20 IL-17+ cells were analyzed in the tracheas of two mice (from two independent experiments).