Table 1.
Quantitative analysis of CD3+ and CD3− cytokine-producing cells in tracheal and lung tissue sections1
| n=3 mice | % of IL-13+ cells (out of total CD3+ T cells) ± SEM | % of IL-17+ cells (out of total CD3+ T cells) ± SEM | # of CD3− cells / total IL-13+ cells (percent) | # of CD3− cells / total IL-17+ cells (percent) |
|---|---|---|---|---|
| Trachea | 1.59 ± 0.11% | 1.06 ± 0.24% | 1/53 (1,89%) | 0/48 (0%) |
| Lung | 0.98 ± 0.30% | 1.93 ± 0.51% | 4/285 (1.4%) | 8/565 (1.42%) |
C57Bl/6 mice were treated with HDM/CT o.ph. on d0 and received HDM o.ph. challenge on d7. Tracheas and lungs were analyzed on d9. IL-17+ or IL-13+ cells were manually identified and co-staining for CD3 was determined. Tissue samples from 3 mice were analyzed.