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. Author manuscript; available in PMC: 2018 Jan 15.
Published in final edited form as: J Immunol. 2016 Dec 7;198(2):776–787. doi: 10.4049/jimmunol.1601585

Figure 1. Homozygous HAQ human B cells have very low MPYS protein expression compared to the R232 B cells and do not respond to natural CDNs.

Figure 1

A–D. R232/R232, HAQ/HAQ, H232/H232 and HAQ/H232 human B cells from indicated ethnic groups were lysed in RIPA buffer and probed for MPYS expression using the rabbit anti-mouse MPYS Ab (n>3). E&G. R232/R232, HAQ/HAQ, H232/H232 and HAQ/H232 human B cells from indicated ethnic groups were activated with 2′3′-cGAMP (10μg/ml) for 5 h as described in Materials and Methods. Nuclear fractions were isolated. Samples were run on a SDS-PAGE gel and probed with the indicated Abs (n=3). F&H. Human IFNβ was measured in cell supernatant from E&G by ELISA (n=3). I. R232/R232, HAQ/HAQ human B cells from indicated ethnic groups were activated with 2′3′-cGAMP (10μg/ml) for 5 h as in E&G. Cells were lysed in the RIPA buffer. Whole cell lysate (WCL) were run on a SDS-PAGE gel and probed with the indicated Abs (n=2). Graph present means ± SEM from three independent experiments. The significance is represented by an asterisk, where p<0.05. n.s, nonspecific. n.d, not detected.