Skip to main content
. Author manuscript; available in PMC: 2018 Jan 15.
Published in final edited form as: J Immunol. 2016 Dec 14;198(2):640–644. doi: 10.4049/jimmunol.1601150

Figure 3.

Figure 3

Mast cells require glycolysis for Ag-induced degranulation and cytokine production. (A–B) WT BMMCs were loaded with Lysotracker Deep Red and sensitized with IgE prior to stimulation with Ag alone, Ag together with DMSO or the indicated concentrations of DCA. Degranulation was assessed by flow cytometry analysis of loss of positive Annexin V staining and decreased Lysotracker staining. A representative degranulation assay is shown in panel A, and the combined results of three experiments are shown in panel B. (C) BMMCs generated from Nur77GFP Tg mice were sensitized with IgE and stimulated with antigen, plus varying concentrations of DCA, or with vehicle control. Nur77GFP expression was measured as an indication of antigen-induced FcεRI activation. Line colors correspond to the bars in panel D. (D) IL-6 release was analyzed by ELISA of culture supernatant harvested after six hours of stimulation. Results are representative of three independent experiments. *p<0.05; **p<0.005.