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. Author manuscript; available in PMC: 2018 Jan 9.
Published in final edited form as: Dev Cell. 2016 Dec 29;40(1):53–66. doi: 10.1016/j.devcel.2016.12.006

Figure 3. Dsh levels are reduced in APC8 knockdown.

Figure 3

(A) Western blots of GMR-GAL4/+, UAS-APC8-IR/+, dshDshGFP/+ (expressed from endogenous dsh control sequences, Axelrod, 2001) or control eye discs (white-IR) at 27°C, probed for GFP. Left side shows 4 disc pairs, right side 8 disc pairs. APC8-IR52280 and APC8-IR52279 show reduced DshGFP levels as compared to control (white-IR). Loading controls: γ–Tub and CyclinB. (B) Quantification of 3 independent Western blots. DshGFP levels in red, controls grey, *P=0.07, **P=0.0027. Statistical analyses here and in subsequent figures were student t-test, unless indicated. Error bars are Standard Deviation in all figures.