Table 2.
ROS1 IHC positive cases
| Case # | KRAS/EGFR/ALK status | ROS1 IHC - % cells positive | ROS1 IHC - Intensity | ROS1 IHC - H score (% x intensity) | ROS1 FISH |
|---|---|---|---|---|---|
| Retrospective Cohort | |||||
| 1 | KRAS & EGFR unknown/ALK− | 90% | 3+ | 244 | + (single set of split signals) |
| 2–81 | 23 KRAS+, 13 EGFR+, 4 PIK3CA+, 2 MET+, 1 ALK+, 1 BRAF+, 1 FGFR1+, 1 HRAS+, 6 unknown | 5–80% (mean =24) | 1+ | 5–80 (mean=24) | - |
| 82–90 | 3 KRAS+, 5 EGFR+, 0 unknown | 20–78 (mean 40) | 2+ | 40–120 (mean 79) | - |
| Prospective cohort | |||||
| 1 | All Negative | 100% | 3+ | 300 | + (classic split signal pattern) |
| 2 | All Negative | 100% | 3+ | 300 | + (classic split signal pattern) |
| 3 | All Negative | 100% | 3+ | 300 | + (single set of split signals) |
| 4 |
KRAS unknown EGFR−/ALK− |
100% | 3+ | 300 | + (classic split signal pattern) |
| 5 |
KRAS unknown EGFR−/ALK− |
100% | 3+ | 300 | + (isolated 3′ green signal pattern) |
| 6 | All Negative | 100% | 3+ | 300 | + (classic split signal pattern) |
| 7 | All Negative | 90% | 3+ | 270 | + (isolated 3′ green signal pattern) |
| 8 | All Negative | 100% | 3+ | 300 | + (classic split signal pattern) |
| 9 | All Negative | 100% | 3+ | 300 | + (classic split signal pattern) |
| 10 | All Negative | 90% | 3+ | 270 | +(isolated 3′ green signal pattern) |
| 11 | All Negative | 100% | 3+ | 300 | + (classic split signal pattern) |
| 12 | All Negative | 100% | 3+ | 300 | + (isolated 3′ green signal pattern) |
| 13 |
KRAS unknown EGFR−/ALK− |
100% | 3+ | 300 | − (isolated 5′ red signal pattern) |
| 14 | All Negative | 25% | 3+ | 75 | - |
| 15 | All Negative | 80% | 3+ | 280 | - |
| 16 | EGFR+/KRAS−/ALK− | 33% | 3+ | 99 | - |
| 17 | EGFR+/KRAS−/ALK− | 20% | 2+ | 40 | - |
| 18 |
KRAS unknown EGFR−/ALK− |
30% | 3+ | 90 | - |
| 19 |
KRAS unknown EGFR−/ALK− |
20% | 3+ | 60 | - |