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. Author manuscript; available in PMC: 2018 Jan 15.
Published in final edited form as: J Immunol. 2016 Dec 16;198(2):623–628. doi: 10.4049/jimmunol.1601686

Figure 2. A2aR activation reduces Tfh and GC-Tfh differentiation.

Figure 2

2W1S:I-Ab tetramer-binding T cells were recovered from the spleen and LNs of 2W1S-PE immunized WT B6 mice after 7d of treatment with either CGS or vehicle alone (PBS). (A) FR4 and CXCR5, (B) CD73 and CXCR5, (C) PD-1 and CXCR5, and (D) Bcl6 and CXCR5 staining in 2W1S:I-Ab-specific CD4 T cells from CGS- or PBS-treated immunized mice. (E) Aggregate numbers of Bcl-6hi CXCR5hi GC-Tfh, Bcl-6lo CXCR5lo Tfh, and Bcl-6– CXCR5– non-Tfh cells that bind the 2W1S:I-Ab tetramer. Data are representative of three independent experiments (n=8-9 mice). *P < 0.05, **P < 0.01, and ***P < 0.001