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. Author manuscript; available in PMC: 2018 Jan 15.
Published in final edited form as: J Immunol. 2016 Dec 16;198(2):623–628. doi: 10.4049/jimmunol.1601686

Figure 3. A2aR inhibition of GC-Tfh differentiation is T cell intrinsic.

Figure 3

2W1S:I-Ab tetramer-bound CD4 T cells were enriched from spleen and LNs of CD4-Cre Adora2af/f conditional knock-out (KO) mice as well as non-Cre expressing WT littermates after 2W1S-PE immunization and a 7d course of either CGS or PBS treatment. (A, B) Frequency (A) and number (B) of 2W1S-specific CD44hi Foxp3– CD4 T cell subsets:Bcl6hi CXCR5hi GC-Tfh, Bcl6lo CXCR5lo Tfh, and Bcl-6– CXCR5– non-Tfh cells. (C, D) Frequency (C) and number (D) of Th17 (RORγt+ Tbet–) and Th1 (RORγt– Tbet+) lineage cells within the non-Tfh fraction of 2W1S:I-Ab tetramer-binding CD4 T cells. (E) 2W1S-specific Foxp3+ Treg numbers. Data are representative of three independent experiments (n=8-9). *P < 0.05, **P < 0.01, and ***P < 0.001