Fig. 7.

The effect of CRT on the ratio of F-actin/G-actin and the acetylation of G-actin of HMEC subjected to MR. a The actin stabilization buffer was used to separate soluble G-actin and insoluble F-actin fractions and examined the F-actin/G-actin ratio by Western blotting analysis to assess polymerization/depolymerization of actin in HMEC. The antibody against acetylated lysine was used to detect the acetylation of G-actin fraction. GAPDH was used as a normalization control. b Densitometry was used to quantitate protein levels based from the relative expression of F-actin and G-actin. Statistical significance was measured as standard deviation (SD), and each condition was performed in triplicate (n = 3). *#P < 0.05 vs. control. c Densitometry was used to quantitate protein levels based from the relative expression of G-actin and acetylated lysine. Statistical significance was measured as standard deviation (SD), and each condition was performed in triplicate (n = 3). *#P < 0.05 vs. control. d The results of Co-IP to verify the direct interaction between CRT and actin