Effect of Fabp1 gene ablation (LKO) on ability of high fat diet (HFD) to impact brain protein levels of cytosolic proteins that bind/chaperone ARA and endocannabinoids. All conditions were as in Figure 5 except that western blot analysis was performed as described in Materials and Methods to determine protein levels of: (A) FABP3 (GAPDH), (B) FABP5 (GAPDH), (C) FABP7 (β-actin), (D) SCP-2 (GAPDH), and (E) HSP70 (β-actin). Relative protein levels were normalized to gel-loading control protein (β-actin or GAPDH), values compared to male WT set to 1, results expressed as the relative ratio of each protein in High-Fat/Control diet, and data shown as mean ± SEM (n = 8); *, P < 0.05 for LKO vs WT; #, P < 0.05 for female (F) vs male (M).