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. Author manuscript; available in PMC: 2018 Jan 1.
Published in final edited form as: J Neurochem. 2016 Nov 29;140(2):294–306. doi: 10.1111/jnc.13890

FIGURE 7.

FIGURE 7

Impact of Fabp1 gene ablation on ability of high fat diet to modify brain protein levels of membrane proteins involved in endocannabinoid synthesis and degradation. All conditions were as in Figure 5 except that western blot analysis was performed as described in Materials and Methods to determine protein levels of: (A) NAPEPLD (GAPDH), (B) DAGLα (GAPDH), (C) FAAH (GAPDH), (D) NAAA (β-actin), (E) MGL (GAPDH), and (F) CB1 (GAPDH). Relative protein levels were normalized to gel-loading control protein (β-actin or GAPDH), values compared to male WT set to 1, results expressed as the relative ratio of each protein in High-Fat/Control diet, and results presented as mean ± SEM (n = 8); *, P < 0.05 for LKO vs WT; #, P < 0.05 for female (F) vs male (M).