Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Jan 11;7:690. doi: 10.3389/fimmu.2016.00690

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2017 Jiang, Jiang, Chen, Lai, Wei, Li, Lin, Wang, Wu, Liang, Liu, Peng, Yu, Weng, Du, Pei, Liu, Yao, Xue and Li.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Construction of chimeric anti-glypican 3 (GPC3) vectors and generation of GPC3-chimeric antigen receptor (CAR) T cells. (A) Schematic representation of a lentiviral vector encoding the signal peptide, anti-GPC3 scFv, CD28 transmembrane domain, 4-1BB costimulatory endodomain, and CD3ζ signaling domain along with eGFP using 2A. (B) A representative of GPC3-CARs expression on human T cells transduced with lentivirus was analyzed using flow cytometry, which detected eGFP at days 7 and 14. (C) Transduction efficiency. Results represent mean ± SD of six individual experiments. No difference was detected between the percentages of GPC3-CAR T and Control CAR T. (D) The expansion of transduced T cells in vitro from day 0 to 21. Results represent mean ± SD of three individual experiments.