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Cytotoxicity of glypican 3 (GPC3)-chimeric antigen receptor (CAR) T cells against targeted cells in vitro. (A) Cytotoxicity of GPC3-CAR T cells was assayed by coincubation with Huh-7-GL (GPC3+), HepG2-GL (GPC3+), and A549-GL (GPC3−) cells at E:T ratios ranging from 1:3 to 5:1. (B) Serial killing of GPC3-CAR cells against Huh-7-GL and HepG2-GL cells at E: T ratios of 1:1 in indicated durations. Results represent mean ± SEM.
