Fig. 5.

Glial Kir5.1 expression is reduced in the absence of Kir4.1 subunit. Immunolabelling for Kir5.1 was determined in optic nerve explants cultures, comparing wild-type mice (A, Kir4.1^+/+) with Kir4.1 knock-out mice (B, Kir4.1^−/−), and following transfection with scrambled shRNA (C) or Kir4.1 shRNA (D); transfected cells were identified by the expression of GFP (appears green) and insets demonstrate Kir4.1 expression in controls (Ai, Ci) and complete ablation in Kir4.1^−/− mice (Bi) and Kir4.1 shRNA (Di). Scale bars 10 μm. Quantification of expression of Kir4.1 (E) and Kir5.1 (F) in Kir4.1^+/+, Kir4.1^−/−, scrambled control and Kir4.1shRNA glia; analysis was performed on 10–12 cells in each group, and data are expressed as mean ± SEM number of voxels per µm³, ***p < 0.001, one-tailed t test