Fig. 7.

Reduction of Kir5.1 in oligodendrocytes and myelin in the absence of Kir4.1. Immunolocalization of Kir5.1 with myelin basic protein, MBP (A, B) and the oligodenrocyte marker APC/CC1 (C–F), in brain tissue from wild-type Kir4.1^+/+ mice (A, C, E) compared to Kir4.1^−/− knock-out mice (B, D, F). Scale bars 20 μm. Western blot analysis of Kir5.1 from total lysates of optic nerve (G) and brain (H) from wild-type Kir4.1^+/+ and Kir4.1^−/− knock-out mice, and mean (±SEM) integrated density normalised against β-actin (I, n = 3, **p < 0.01; ***p < 0.001, one-tailed t test)