Table 1.
Antibody specifications
| Raised against | Raised in | Protein cc. of stock solution | Dilution | Source | Catalog number | Lot number | Characterized |
|---|---|---|---|---|---|---|---|
| VGLUT3 | Rabbit | 1 μg/μl | 1:500 | Synaptic Systems | 135203 | Szonyi et al. (2014) | |
| NeuN | Mouse | 1 mg/ml | 1:500 | Chemicon | MAB 377 | LV 1359479 | Mullen et al. (1992) |
| Serotonin | Rat | Not available | 1:500 | Merck Millipore | MAB 352 | 2168248 | Amilhon et al. (2010) |
| Serotonin | Rabbit | 25 μg/ml | 1:10000 | ImmunoStar | 20080 | 1431001 | Fox and Deneris (2012) |
| TPH | Mouse | 1:3000 | Sigma-Aldrich | T0678 | Calizo et al. (2011) |
| Conjugated with | Raised in | Raised against | Dilution | Source | Catalog number | Molecule | |
|---|---|---|---|---|---|---|---|
| Alexa 594 | Donkey | Mouse | 1:500 | Life technologies | A-21203 | Full IgG | |
| Alexa 647 | Chicken | Rat | 1:500 | Life technologies | A-21472 | Full IgG | |
| Alexa 647 | Donkey | Rabbit | 1:500 | Jackson immuno-research | 711-605-152 | Full IgG | |
| Cy3 | Donkey | Rabbit | 1:500 | Jackson immuno-research | 711-165-152 | Full IgG | |
| Alexa 488 | Donkey | Mouse | 1:500 | Life technologies | A21206 | Full IgG | |
| Alexa 488 | Donkey | Rabbit | 1:500 | Life technologies | A 21202 | Full IgG | |
| DAPI | – | – | 1:10.000 | Sigma-Aldrich | D9564 | – |