Table 3.
Number of cells in median raphe
| The number of cells in median raphe in eight mice | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Labelled cell types | GM1 | GM2 | GM3 | GM4 | WT | PM1 | PM2 | PM3 | Mean |
| Non-neuronal cells, only DAPI positive | 11269 | 10449 | 10672 | 11928 | – | – | – | – | 11079 |
| All neurons (calculated, using all mice, from the mean number of all labelled neurons) | 8160 | ||||||||
| All 5-HT positive cells, without VGLUT3 labeling—abbreviated as “SO” | 1310 | 515 | 573 | 662 | 640 | 807 | 351 | 697 | 695 |
| And from these, ePET positive cells are: | – | – | – | – | – | 380 | 240 | 378 | 333 |
| All VGLUT3 positive cells, without 5-HT labeling—abbreviated as “GO” | 1840 | 2078 | 2209 | 2055 | 2041 | 2209 | 2591 | 1957 | 2123 |
| And from these, ePET positive cells are: | – | – | – | – | – | 906 | 886 | 959 | 917 |
| All 5-HT and VGLUT3 double positive cells—abbreviated as “SG” | 627 | 890 | 904 | 940 | 1408 | 1427 | 1014 | 1126 | 1042 |
| And from these, ePET positive cells are: | – | – | – | – | – | 1002 | 705 | 891 | 866 |
| All 5-HT and/or VGLUT3positive cells | 3655 | 3450 | 3426 | 3620 | 4089 | 4862 | 3957 | 3780 | 3855 |
| All VGAT positive cells, without 5-HT and VGLUT3 labeling | 3105 | 3190 | 2619 | 3222 | – | – | – | – | 3034 |
| All VGAT and VGLUT3 double positive cells, without 5-HT labeling | 35 | 149 | 93 | 65 | – | – | – | – | 86 |
| All NeuN positive cells (triple negative), without 5-HT, VGLUT3 and VGAT labeling | 597 | 712 | 1632 | 1782 | – | – | – | – | 910 |
| All ePET positive cells, without 5-HT and/or VGLUT3 labeling | – | – | – | – | – | 68 | 451 | 296 | 271 |