Fig. 7.

Concentration- and time-dependent effect of ATX II on gene expression of γGCL and Nrf2 in HT29 cells. Impact of ATX II on relative gene transcription of phase 2 enzyme γGCL (white background) and transcription factor Nrf2 (black background) in HT29 cells after 3 h (a) and 24 h (b). 0.1 % EtOH served as solvent control, 0.5 µM CDDO-Im and 200 µM tBHQ as positive controls. Depicted are mean $2^{-\mathrm{\Delta }\mathit{\Delta }{C}_{\text{T}}}$ ±SEM as fold transcription of 3–5 independent experiments compared to solvent control. Significance levels indicated as asterisk refer to comparison with solvent control (EtOH; *p < 0.05, **p < 0.01, ***p < 0.001)