Table 1.
Sequence of primers used in real-time PCR, amplicon sizes, annealing temperatures, and the amplification efficiencies
| Target's Accession No. | Sequences | Amplicon length (bp) | Annealing Tm(oC)/t(s) | PCR efficiency |
|---|---|---|---|---|
| SERPINB2 NM_002575 | F: 5′ GAATGCTGTCTACTTCAA 3′ | 147-i | 55/15 | 0.99 |
| R: 5′ TCTTCTATGTATCCAATGTT 3′ | ||||
| SLC7A5 NM_003486 | F: 5′ G-GTGATGTGTCCAATCTA 3′ | 116 | 56/15 | 1.00 |
| R: 5′ AAGTAATTC-CATCCTCCATA 3′ | ||||
| SLC14A1 NM_015865 | F: 5′ GACATTACAATCCATTCT 3′ | 140-i | 52/15 | 0.96 |
| R: 5′ ATTATCACAGCCATAGAT 3′ | ||||
| CCNE2 NM_057749 | F: 5′ GTTCTTCTACCTCAGTATTCTC 3′ | 114-i | 55/10 | 0.98 |
| R: 5′ AGCAGCAGTCAGTATTCT 3′ | ||||
| TIPARP NM_015508 | F: 5′ CTGTCTTGCCATATCATT 3′ | 144 | 55/10 | 0.96 |
| R: 5′ ATTCTTGTCC-TCCATACT 3′ | ||||
| STC2 NM_003714 | F: 5′ CAACTCTTGTGAGATTCG 3′ | 110-i | 58/15 | 0.91 |
| R: 5′ TACATTTCAAGGCGTCTT 3′ | ||||
| SCG5 NM_003020 | F: 5′ CAAGAAACTCCTTTACGA 3′ | 138-i | 56/15 | 0.93 |
| R: 5′ TCCTTATCCTCATCTGAA 3′ | ||||
| TMEM156 NM_024943 | F: 5′ G-TTCTTATCAGGAGAGGAT 3′ | 123 | 56/15 | 0.94 |
| R: 5′ ATGACAGGTAGTGTTATATTC 3′ | ||||
| GSTA2 NM_000846 | F: 5′ CCACTACTCCAATATACG 3′ | 165-i | 58/15 | 0.94 |
| R: 5′ CCATCAATCTCAACCATT 3′ |
The hyphen in the primer sequence denotes the exon/exon boundary. Letter “i” after the amplicon length indicates that exon/exon boundary was inside the amplified sequence