Table 2.

Summary of the experimental design applied to analyze gene expression by microarray and qPCR

Type of cells	Transfected vector	Treatment
HepaRG undifferentiated	Negative control	DMSO
HepaRG undifferentiated	Negative control	BNF
HepaRG undifferentiated	AhR(−)	DMSO
HepaRG undifferentiated	AhR(−)	BNF
HepaRG differentiated	Negative control	DMSO
HepaRG differentiated	Negative control	BNF
HepaRG differentiated	AhR(−)	DMSO
HepaRG differentiated	AhR(−)	BNF

The negative control shRNA is a scrambled artificial sequence which does not match any human gene. The AhR(−) shRNA is a sequence which decreases the expression of AhR mRNA by RNA interference. Specimens in italic were analyzed only by qPCR