. 2016 Oct 28;425(1):59–75. doi: 10.1007/s11010-016-2862-3

Table 8.

Validation of selected microarray-based genes expression data by real-time quantitative PCR

	Relative expression of mRNA ± SD
Type of cells	HepaRG undifferentiated				HepaRG differentiated
Transfected vector	Negative control		AhR(−)		Negative control		AhR(−)
Treatment	Solvent	BNF	Solvent	BNF	Solvent	BNF	Solvent	BNF
Gene
CYP1A1	0.405 ± 0.2113	100.0 ± 57.28^a	4.091 ± 2.582	49.51 ± 42.20	3.132 ± 1.569	32.22 ± 14.21^a	8.803 ± 6.778	14.67 ± 12.42
GSTA2	70.44 ± 3.862	17.62 ± 5.749^a	34.94 ± 3.833	13.91 ± 4.109^a	52.89 ± 33.33	13.75 ± 5.733	100.0 ± 80.27	30.93 ± 13.82
SERPINB2	1.205 ± 0.6849	100.0 ± 10.22^a	1.234 ± 1.032	20.73 ± 13.18^b	1.173 ± 0.1412	33.80 ± 11.94^a	0.2250 ± 0.1469	2.713 ± 1.336^b
SLC7A5	6.274 ± 1.461	100.0 ± 22.94^a	2.963 ± 0.5229	18.74 ± 10.17^b	2.547 ± 0.6880	89.42 ± 28.47^a	1.687 ± 0.5035^c	7.641 ± 2.617^ab
SLC14A1	11.15 ± 0.1337	100.0 ± 34.49^a	2.307 ± 1.731	3.675 ± 3.376^b	52.47 ± 36.10	78.07 ± 19.72	4.562 ± 3.840	2.147 ± 0.7728^b
CCNE2	18.77 ± 9.006	53.92 ± 16.49^a	24.98 ± 11.91	100.0 ± 14.59^ab	52.82 ± 6.010	71.14 ± 11.51	35.74 ± 8.194	32.58 ± 17.95^b
TIPARP	13.22 ± 2.026	98.99 ± 10.46^a	11.67 ± 1.943	18.42 ± 8.759^b	24.62 ± 2.026	100.0 ± 24.18^a	24.45 ± 3.926	24.22 ± 4.037^b
STC2	35.39 ± 16.29	100.0 ± 22.33^a	43.16 ± 21.43	62.09 ± 21.18	18.25 ± 12.41	92.41 ± 78.18	17.65 ± 3.669	57.64 ± 49.78
SCG5	12.95 ± 8.600	100.0 ± 47.09^a	1.514 ± 0.6559	17.69 ± 15.42^b	2.632 ± 1.659	9.453 ± 5.483	0.1560 ± 0.1291	0.9109 ± 0.6250^b
TMEM156	12.75 ± 7.720	100.0 ± 22.84^a	4.393 ± 1.505	21.13 ± 11.91^b	1.233 ± 0.0565	7.672 ± 0.4095^a	0.4391 ± 0.1383^c	1.158 ± 0.2322^ab
AhR	37.51 ± 9.057	33.92 ± 4.433	7.732 ± 1.840^c	9.052 ± 3.721^c	100.0 ± 23.76	81.13 ± 10.21	11.11 ± 4.527^c	9.161 ± 0.9119^c

^aSignificantly different from solvent-treated cells

^bSignificantly different from BNF-treated negative control transfected cells

^cSignificantly different from negative control transfected cells