Figure 2.
YM155 does not bind DNA (A) Viscometric titrations of calf thymus DNA with ethidium bromide (EtBr), Netropsin (Net), and YM155. The data are the average of three separate readings. Where η and η0 represent the viscosity of the solution containing ligand-DNA complex and free DNA respectively. (B) Circular dichroism spectra of YM155 with hairpin duplex DNA at different DNA-drug ratios. The experiments were conducted in Tris-HCl buffer (50 mM Tris-HCl, 100 mM NaCl, 1mM EDTA, pH = 7.4) at 25 °C. (C–F) Energy minimized structures of YM155 at the 6–31G* (p,d) level of theory by Spartan 10 software. YM155 is represented as a ball and stick model (C), and space filling model with front (D), back (E), down (F) side view with carbon in grey, hydrogen in yellow, oxygen in red and nitrogen in blue.