Figure 6.

Evaluation of trauma localization. Trauma is limited to the injured region by histologic analysis and Evans' blue quantification. A: Near-infrared imaging of fixed brain slices shows an intense signal in mild traumatic brain injury (mTBI) (right) but not tail trauma mice (left), where it was limited to the vasculature. B: Hematoxylin and eosin staining confirms localization of the damage. C: Quantification of albumin extravasation by near-infrared scans shows that mTBI mice have higher signals across the measured sections. D: Near-infrared imaging indicates the presence of Evans' blue leakage of tail trauma (TT) mice (left) whereas mTBI mice (right) have intensities limited to vascular regions. E: Hematoxylin and eosin sections confirm localization of damage in the tail. F: Quantification of Evans' blue fluorescence shows increased signal in the tails of TT mice. Data are shown as means ± SEM (C and F). n = 4 per group.