Figure 4.

Cytokine analysis in the supernatants derived from ANT₁ 21-40–stimulated cultures. Lymph node cells obtained from ANT₁ 21-40–immunized mice were stimulated with or without ANT₁ 21-40 or RNase 43-56 (control). Supernatants collected on day 3 after stimulation were analyzed using beads conjugated with cytokine-capture antibodies and detection antibodies by cytometric bead array, as described in Materials and Methods. Values obtained from four individual experiments are shown. Data are expressed as means ± SEM. ^∗P ≤ 0.05. IFN-γ, interferon-γ; Th, T helper; TNF-α, tumor necrosis factor-α.