Table 1.

Antibodies Used for IHC Analysis

Antibody	Clone	Vendor	Working concentration	Antigen retrieval condition	Antibody incubation condition
MHL1	M1	Ventana Medical Systems (Tucson, AZ)	1.4 μg/mL	64 Minutes at 95°C; Ultra Cell Conditioning Solution 1 (Ventana Medical Systems)	36 minutes at 37°C
MSH6	SP93	Cell Marque (Rocklin, CA)	1:50 dilution	52 Minutes at 95°C; Ultra Cell Conditioning Solution 1 (Ventana Medical Systems)	1 hour at 37°C
MSH2	FE11	Dako (Carpinteria, CA)	1:10 dilution	60 Minutes at 95°C; Retrieval Bond Epitope Retrieval Solution 2 (Leica Microsystems, Buffalo Grove, IL)	15 minutes at room temperature
PMS2	A16-4	BD Biosciences (San Jose, CA)	1:50 dilution	40 Minutes at 95°C; Retrieval Bond Epitope Retrieval Solution 2 (Leica Microsystems)	15 minutes at room temperature

MLH1, mutL homolog 1; MSH2, mutS homolog 2; MSH6, mutS homolog 6; PMS2, PMS1 homolog 2.