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. 2017 Jan;187(1):146–155. doi: 10.1016/j.ajpath.2016.09.012

Figure 4.

Figure 4

Cad-11 expression in isolated human primary PSCs and pancreatic cell lines. A: Quantitative PCR analysis of Cad-11 mRNA levels expressed as fold change compared with PSC-N or HPDE6 control. B: Western blot analysis of Cad-11 protein levels. Densitometric quantification of data is shown in the graph as fold change using PSC-N or HPDE6, respectively, as control. The arrowheads indicate the bands that represent the pro- and mature forms of the Cad-11 protein. C: Flow cytometric analysis of Cad-11 in PSC-N and PSC-CP. Overlay of the histogram is shown. D: Flow cytometric analysis of Cad-11 in BxPC-3 and HDPE6 cells. E: Flow cytometric comparison of Cad-11 between PSC-N and PSC-CP or HPDE6 and BxPC-3. Data are expressed as fold change compared with PSC-N or HPDE6 control, and the error bars represent SD (A, B, and E). n = 3 separate experiments (A, B, and E). ∗P < 0.05, ∗∗P < 0.01. APC-A, allophycocyanin area; Cad-11, cadherin-11; CP, chronic pancreatitis; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; HDPE6, immortalized human pancreatic epithelial cell; PSC, pancreatic stellate cell; PSC-CP, PSC isolated from CP tissue; PSC-N, primary PSC isolated from normal pancreas; α-SMA, α-smooth muscle actin.