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. 2017 Jan;187(1):146–155. doi: 10.1016/j.ajpath.2016.09.012

Figure 5.

Figure 5

Knockdown of cad-11 reduced pancreatic cancer cell migration. A: Quantitative PCR analysis of Cad-11 mRNA levels in BxPC-3 cells transfected with control (siControl) or Cad-11–specific siRNAs (siCad-11 H3 and H4). B: Western blot analysis of Cad-11 protein levels in BxPC-3 cells transfected with control or Cad-11–specific siRNAs. The graph shows densitometric quantification of Cad-11 levels normalized to GAPDH. C: Western blot analysis of Cad-11 and GAPDH protein levels in BxPC-3 cells after 24-hour incubation in medium containing 10% FBS, SF, or SF containing 5 ng/mL TGF-β. D: Microscopic image of migrated BxPC-3 cells in Transwell. BxPC-3 cells were transfected with control or Cad-11–specific siRNA (siCad-11 H3). The concentration of TGF-β is indicated. E: Quantification of Transwell migration as shown in D by crystal blue staining. F: MTT assay of BxPC-3 cell viability 72 hours after transfection with control or Cad-11–specific siRNA (siCad-11 H3). Data are expressed as fold change compared with siControl, and the error bars represent SD (A, B, E, and F). n = 3 separate experiments (A, B, E, and F). ∗P < 0.05, ∗∗P < 0.01. Cad-11, cadherin-11; CTRL, control; FBS, fetal bovine serum; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; SF, serum-free medium; TGF-β, transforming growth factor β.