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. 2017 Jan;187(1):214–224. doi: 10.1016/j.ajpath.2016.09.010

Figure 5.

Figure 5

Kinesin-derived angiogenesis inhibitor (KAI) inhibits endothelial cell migration, capillary network formation, and sprouting. A and B:In vitro two-dimensional capillary network formation. Human primary umbilical vein endothelial cells (HUVECs) were treated with phosphate-buffered saline (PBS) vehicle, synthetic peptides KAI, or CT23 at the indicated doses and tested for capillary network formation in Matrigel in the presence of 2.2 nmol/L vascular endothelial growth factor (VEGF). C–E:In vitro three-dimensional sprouting assay in fibrin gel supplemented with VEGF (2.2 nmol/L). HUVECs were treated PBS, KAI, or CT23 1 μmol/L as indicated. Data are expressed as means ± SEM (B, D, and E). n = 10, 4, 6, 4, 4, 3, and 3 in the groups, respectively (B); n = 11, 9, and 6 for the control, KAI, and CT23 treated groups, respectively (D and E). ∗P < 0.05 (one-way analysis of variance). Scale bars: 200 μm (A); 50 μm (C).