TABLE 1 .

List of ppGalNAc-T2 substrate candidate proteins identified from the jacalin affinity purification^a

Identified protein	Accession no.	Predicted O-GalNAc	Peptide count
			WT	Δt2	WT/Δt2
CST1	TGME49_264660	197	96	7	13.71
Hypothetical	TGME49_313270	67	42	17	2.55
Hypothetical	TGME49_230940	265	39	3.5	11.14
MIF4G domain containing	TGME49_269180	153	31	7.5	4.07
Hypothetical	TGME49_228120	117	25	5	4.90
GYF domain containing	TGME49_204160	37	14	5.5	2.55
VSP13	TGME49_291180	239	14	5.5	2.55
GYF domain containing	TGME49_298610	98	12	0	NA
GRA7	TGME49_203310	1	9.5	3	3.17
Kelch repeat containing	TGME49_229000	11	9.5	2	4.75
Sec7 domain containing	TGME49_232190	139	9.5	3	3.17
GRA3	TGME49_227280	4	8.5	3	2.83
Hypothetical	TGME49_313370	74	8	2	4.00
Hypothetical	TGME49_215910	142	7.5	0	NA
Hypothetical	TGME49_288460	53	7	0	NA
Hypothetical	TGME49_287480	251	6.5	0	NA
Perforin-like protein PLP1	TGME49_204130	26	6	1	6.00
LsmAD domain containing	TGME49_231440	66	6	0	NA
Pyrroline-5-carboxylate reductase	TGME49_236070	9	6	2	3.00
Thioredoxin reductase	TGME49_309730	22	6	2	3.00
Hypothetical	TGME49_311210	71	6	0	NA
Lysine decarboxylase family	TGME49_236570	7	5.5	0	NA

^aThe list is sorted by the number of peptides detected in wild type (WT). Predicted O-GalNAc, number of glycosylation sites predicted by NetOGlyc 4.0; WT and ppGalNAc-Δt2, normalized peptide count detected in mass spectrometry; WT/Δt2, ratio of normalized peptide count between wild type and Δt2 sample (NA represents no count detected in Δt2 sample). The list was filtered with the semiquantitative criteria (proteins that have more than 5 peptides mapped in wild type with more than a 2.5-fold-increased detection in wild type over Δt2 sample).