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. 2017 Jan 18;10(1):23–29. doi: 10.18240/ijo.2017.01.04

Figure 2. Phase-contrast microscopic changes (×200) in morphology during induction.

Figure 2

A-F: EN derived orbital ADSCs; G-L: EX derived orbital ADSCs. A&G: Before induction, the cells are spindle shape. Connections between cells were scarcely detected; B&H: After 7d in 1×NDI medium; C&I: After 7d in 10×NDI medium; D&J: After 7d in RP medium, the cells lost the spindle shape and formed round shape gradually; E&K: After 14d in RP medium with 100mM Vitamin A for 24h; synapses were formed widely between cells. Cytotoxic effect was detected at this point. K: Black arrow pointed to an EX orbital ADSC showing tubular, rod-like structure. F&L: After 14d in RP medium with 100mM DMSO for 24h. These significant changes of morphology were not detected.