Figure 2.

Hypoxic stress induces intracellular ROS but does not affect adipose tissue-derived mesenchymal stromal cell viability. (a) Intracellular ROS were detected with CM-H₂DCFDA (10 μM for 20 min) and analysed by flow cytometry. (b) Intracellular NO levels were evaluated by flow cytometry using DAF-FM diacetate (2 μM for 30 min, followed by a 30 min incubation in fresh medium for complete deesterification). (c) Cell viability was determined by analysing AnnexinV and propidium iodide binding by flow cytometry. Data represent the mean + standard deviation (n = 3), ^∗ p < 0.05.