Figure 4.

dNek2 Mediates the Differentiation Impairment Caused by Cdc16 Depletion

(A–D) Adult eyes of the indicated genotypes. ey>dNek2RNAi1 did not show any detectable defects (B). However, it fully rescued the differentiation failure phenotype of ey>cdc16RNAi1 (C) and (D).

(E–J) Eye imaginal discs of the indicated genotypes stained for DNA (blue), CycB (green), PH3 (red), and ELAV (white). Anterior to the left. The eye discs of ey>dNek2RNAi (F–F₂) are comparable with the control (E–E₂). However, dNek2RNAi1 fully restored photoreceptor differentiation and the uniform MF progression in the eye discs of ey>cdc16RNAi1 (H–H₂) compared with (G–G₂), without restoring the increased number of mitotic cells (G₁, H₁). Scale bars, 50 μm. Relative differentiated regions were quantified and are presented in a scattered dot plot (I). Each dot represents an individual measurement and horizontal bars the mean values (n = 15–30). The numbers of mitotic cells per eye disc were quantified and the mean values are presented in a bar graph (J). The error bars indicate SD (n = 6–22). ^∗p < 0.05, ^∗∗∗p < 0.0001. n.s., no statistical difference. See also Figure S5.