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. 2017 Jan 11;7:40467. doi: 10.1038/srep40467

Figure 1. Aβ (1–42) accumulation in the culture supernatants (CS) from HBMECs exposed to HIV-1 gp120, NT and METH.

Figure 1

The diagram shows results of direct anti-Aβ (1–42) ELISA measurements of the undiluted CS samples collected from HBMECs cultures (n = 6 per group) treated with gp120 (50 ng/ml), NT (10 μM) and METH (50 nM) either alone or in combination of them [METH (50 nM) + gp120 (50 ng/ml) or NT (10 μM) + gp120 (50 ng/ml)] for 24 h. The freshly prepared Aβ (1–42) stock was serially diluted with cell culture growth medium to generate standard ELISA calibration curves. Data were presented as mean values ± SEM. **Marks significant (P < 0.01) differences in extracellular Aβ (1–42) accumulation.