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. 2017 Jan 11;7:40413. doi: 10.1038/srep40413

Figure 6. MiR-218 inhibits heroin-induced CPP by directly targeting MeCP2.

Figure 6

(a) Schematic representation of miR-218 sequence and its target sequences within the 3′ UTR of Mecp2. The seed sequence is indicated by bold letters, while the pairing sequence of miR-218 is in red bold letters. (b) Relative luciferase activity with the Mecp2 3′ UTR or mutated 3′ UTR was determined in 293 T cells co-transfected with scramble or miR-218 mimic. (n = 6 for each group, ***P < 0.001 vs. scramble with Mecp2 3′ UTR). (c) Relative Mecp2 level with LV-control or LV-miR-218 infection in C6 cell (n = 6 for each group, **P < 0.01 vs LV-control). (d) CPP score of Mecp2+/y and Mecp2308/y mice in pre-test and test sessions (Mecp2+/y, n = 9, Mecp2308/y, n = 7, *P < 0.05 vs. Mecp2+/y mice during test session). Data are shown as mean ± s.e.m.