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. 2017 Jan 11;7:40400. doi: 10.1038/srep40400

Figure 5. Effect of knockdown or overexpression of CXADR on 6G10A anti-tumor activity in vivo.

Figure 5

(a) CXADR expression in DU-145 cells transfected with control vector or an shRNA expression vector. The gels have been run under the same experimental conditions and cropped to show protein bands corresponding to CXADR or tubulin as indicated. (b) DU-145 cells expressing a control vector (Vector cont) or CXADR shRNA vector (sh CXADR) cells were injected subcutaneously into male nude mice. Antibodies (250 μg/day) were administered intravenously 1, 7, and 14 days after the cancer cell injection. Mice were sacrificed 21 days after the cancer cell injection, and the tumors were excised and weighed. The values are means ± SEM (n = 3). **P < 0.01 versus the control values. (c) CXADR expression in MKN-7 cells expressing a control vector (Vector cont), CXADR, CXADR iso5, or mutant CXADR (mt). The gels have been run under the same experimental conditions and cropped to show protein bands corresponding to CXADR or tubulin as indicated. (d) MKN-7 cells expressing a control vector (Vector cont), CXADR, or CXADR iso5 were injected subcutaneously into female nude mice. Antibodies (250 μg/day) were administered intravenously 1, 7, and 14 days after the cancer cell injection. Mice were sacrificed 21 days after the cancer cell injection, and the tumors were excised and weighed. The values are means ± SEM (n = 5). **P < 0.01 versus the control values.