Skip to main content
. 2017 Jan 11;7:40400. doi: 10.1038/srep40400

Figure 6. Effect of anti-CXADR antibody 6G10A on ADCC and CDC.

Figure 6

(a) For ADCC activity, DU-145 cells were incubated with splenocytes from male nude mice in the presence of the indicated antibodies at 100 μg/ml for 4 h. For CDC activity, DU-145 cells were incubated with 10% rabbit complement in the presence of the indicated antibodies for 4 h. Cell lysis was determined using calcein AM. The values are means ± SEM (n = 3). *P < 0.05 and **P < 0.01 versus the control values. (b) DU-145 cells were injected subcutaneously into male nude mice. To deplete NK cells in the mice, anti-asialo GM1 antibodies (100 μg/day) were administered intravenously −1, 6, and 13 days after the cancer cell injection. Anti-CXADR antibody 6G10A (250 μg/day) was administered intravenously 1, 7, and 14 days after the cancer cell injection. The mice were sacrificed 21 days after the cancer cell injection, and the tumors were excised and weighed. The values are means ± SEM (n = 5). *P < 0.05 and **P < 0.01 versus the control values.