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. 2017 Jan 11;7:40357. doi: 10.1038/srep40357

Table 2. Catalytic parameters of wild-type AIM-1 and selected mutants. Parameters were determined from reactions with three different substrates.

  Ampicillin
 Cefoxitin
 Imipenem
kcat (s−1) KM (μM) kcat/KM (s−1 μM−1) kcat (s−1) KM (μM) kcat/KM (s−1 μM−1) kcat (s−1) KM (μM) kcat/KM (s−1μM−1)
AIM-1 410 ± 30 110 ± 21 3.8 117 ± 9 283 ± 37 0.4 1405 ± 242 289 ± 66 5.0
W38C 131 ± 11 92 ± 21 1.5 13 ± 2 12 ± 3 1.2 254 ± 41 157 ± 30 1.6
F119M 2327 ± 754 388 ± 163 6.2 122 ± 19 30 ± 11 4.3 1240 ± 400 279 ± 102 4.6
S221A 251 ± 62 434 ± 190 0.6 54 ± 3 19 ± 2 3.0 23 ± 4 70 ± 12 0.3
S221E 0.4 ± 0.2 57000 ± 25000 ~0 ~0 1018 ± 58 ~0 ND ND ND
#5–1 382 ± 3 92 ± 7 4.2 60 ± 2 17.6 ± 2 3.5 82.9 ± 7 11 ± 1 7.6
#5–2 481 ± 35 206 ± 49 2.4 79 ± 7 28 ± 2 2.8 141 ± 20 40 ± 5 3.5
#5–3 440 ± 17 140 ± 12 3.2 93 ± 5 36 ± 5 2.6 196 ± 49 87 ± 34 2.4
#5–19 2335 ± 361 1065 ± 216 2.2 352 ± 44 237 ± 44 1.5 40 ± 3 13 ± 2 3.1