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| Evolution Rounds | Methods | Cefoxitin (Agar/Liquid) | Library Size (n) (Primary/Secondary) |
|---|---|---|---|
| Round 1 | ePCR | 50/100 μg/mL | 4,000/100 |
| Round 2 | ePCR | 60/120 μg/mL | 16,000/100 |
| Round 3 | ePCR | 75/150 μg/mL | 33,000/200 |
| Round 4 | ePCR | 100/200 μg/mL | 10,000/200 |
| Round 5 | eStEP | 125/250 μg/mL | 242,000/300 |
