Table 2.
Fission yeast strains and plasmids
| Strains and plasmids | Genotype and characters | Source or reference |
|---|---|---|
| S. pombe strains | ||
| SP223 | wild type, h − , ade6-216, leu1-32, ura4-294 | Laboratory collection |
| Plasmids | ||
| pYZ1N | Fission yeast expression vector with an inducible nmt1 promoter and a LEU2 selectable marker | [50] |
| pYZ2N | Same as pYZ1N but with a ura4 selectable marker | [50] |
| pYZ3N | Same as pYZ1N but with a 5′ GFP-tag | [50] |
| pYZ1N-PR | Wild type HIV-1 PR gene cloned in pYZ1N Used to test its effect on cell growth and killing |
[32] |
| pYZ1N-M7 | Drug resistant M7 HIV-1 drPR gene cloned in pYZ1N Used to test its effect on cell growth and killing |
This study |
| pYZ1N-M10 | Drug resistant M10 HIV-1 drPR gene cloned in pYZ1N Used to test its effect on cell growth and killing |
This study |
| pYZ1N-M11 | Drug resistant M11 HIV-1 drPR gene cloned in pYZ1N Used to test its effect on cell growth and killing |
This study |
| pYZ2N-PR | Wild type HIV-1 PR gene cloned in pYZ2N Used to test HIV-1 PR proteolytic cleavage |
[32] |
| pYZ2N-M7 | Drug resistant M7 HIV-1 drPR gene cloned in pYZ2N Used to test HIV-1 PR proteolytic cleavage |
This study |
| pYZ2N-M10 | Drug resistant M10 HIV-1 drPR gene cloned in pYZ2N Used to test HIV-1 PR proteolytic cleavage |
This study |
| pYZ2N-M11 | Drug resistant M11 HIV-1 drPR gene cloned in pYZ2N Used to test HIV-1 PR proteolytic cleavage |
This study |
| pYZ3N-GFP-MA-Vpr | GFP is connected to Vpr in pYZ3N by a polylinker containing the PR cleavage MA↓CA site (DSQNY↓PIVQ) | [32] |
| pYZ3N-p6-MA-Vpr | GFP is connected to Vpr in pYZ3N by a polylinker containing the PR cleavage p6 site (DSFNF↓PQIT) | [32] |