Fig. 1.

Identification of PCBP1 as a novel interaction partner of wild type and mutant HSPB1. a Co-immunoprecipitation was performed on patient-derived lymphoblastoid cell lines. Pull-down was directed against endogenously expressed PCBP1 and checked for the presence of endogenous HSPB1. IgG was used as a negative control. b Co-immunoprecipitation was performed on transiently transfected HeLa cell lines, clearly showing the binding of PCBP1 to HSPB1-WT and the increased binding to HSPB1-P182L. Pull down was directed against the V5-tag of the HSPB1 constructs and the presence of PCBP1 was checked with a VSV-tag. EGFP-V5 was used as a negative control. c Calculation of the PCBP1/HSPB1 ratio after quantification of the relative band intensities (n = 3). A One way ANOVA with Holm-Sidak multiple comparison test was performed to indicate the significant increased interaction of HSPB1-P182L with PCBP1. Data are represented as mean values with SD as error bars