Fig. 6.

PCBP1 controls the expression of known neuropathy genes. a Venn diagram showing the overlap between genes identified through RIP sequencing (≥2 fold) and known IPN and dHMN genes. Overlapping genes were confirmed by RNA immunoprecipitation of PCBP1 followed by RT-qPCR on total mouse brain. Raw Ct values originating from mRNA targets when PCBP1 was pulled down were corrected for the pull down of IgG and normalized for the Ct values in the total lysate used for the pull down experiment. These obtained values are shown as input percentage values. b The same approach was done for an additional dataset with all known genes causing HSP. c Co-immunoprecipitation was performed on NSC-34 cells stably transduced with HSPB1 wild type and mutants. Pull-down was directed against endogenous PCBP1 and the presence of exogenous HSPB1 was investigated. d The expression level of a selection of identified PCBP1 targets was checked in stably transduced NSC-34 cell lines by western blot and by RT-qPCR (e). Values are shown as mean with SD as error bar. Multiple t-test with Holm-Sidak as a correction for multiple comparisons was used as statistical test